Tracheal colonization factor A (TcfA) is a biomarker for rapid and specific detection of Bordetella pertussis.

ABSTRACT: Pertussis is a highly contagious disease for which prompt, point-of-care diagnosis remains an unmet clinical need. Results from conventional test modalities (nucleic acid detection, serology, and culture) take hours to days. To overcome this challenge, we identified a new biomarker (tracheal colonization factor A, TcfA) for detection of Bordetella pertussis infection by lateral flow immunoassay (LFIA). We developed a library of 28 epitope-mapped monoclonal antibodies against TcfA and incorporated three antibodies into a LFIA. The LFIA did not cross-react with common bacterial or fungal organisms, but did react with nine distinct B. pertussis strains. The minimal linear epitope sequences targeted by the LFIA were conserved in 98% of 954 B. pertussis isolates collected across 12 countries from 1949-2017. The LFIA's limit of detection was 3.0?×?105 CFU/mL with B. pertussis cells in buffer, 6.2?×?105 CFU/mL with nasopharyngeal washes from a non-human primate model, and 2.3 ng/mL with recombinant TcfA. The LFIA reacted with patient nasopharyngeal swab specimens containing as few as 1.8?×?106 B. pertussis genomes/mL and showed no false-positives. Rapid (

SUBMITTER: Burnham-Marusich AR 

PROVIDER: S-EPMC7490692 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature