WD repeat-containing protein 1 maintains β-Catenin activity to promote pancreatic cancer aggressiveness.

ABSTRACT:

Background

The molecular signature underlying pancreatic ductal adenocarcinoma (PDAC) progression may include key proteins affecting the malignant phenotypes. Here, we aimed to identify the proteins implicated in PDAC with different tumour-node-metastasis (TNM) stages.

Methods

Eight-plex isobaric tags coupled with two-dimensional liquid chromatography-tandem mass spectrometry were used to analyse the proteome of PDAC tissues with different TNM stages. A loss-of-function study was performed to evaluate the oncogenic roles of WD repeat-containing protein 1 (WDR1) in PDAC. The molecular mechanism by which WDR1 promotes PDAC progression was studied by real-time qPCR, Western blotting, proximity ligation assay and co-immunoprecipitation.

Results

A total of 5036 proteins we

SUBMITTER: Li H 

PROVIDER: S-EPMC7492282 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature