Morphological and Molecular Characterization of Toxigenic Aspergillus flavus from Groundnut Kernels in Kenya.

ABSTRACT: Pathogenesis of Aspergillus flavus on important agricultural products is a key concern on human health due to the synthesis and secretion of the hazardous secondary metabolite, aflatoxin. This study identified and further characterized aflatoxigenic A. flavus from groundnuts sampled from sundry shops in Kenya using integrated morphological and molecular approaches. The groundnuts were plated on potato dextrose agar for isolation and morphological observation of A. flavus based on macroscopic and microscopic features. Molecular characterization was done through amplification and comparison of the partial sequence of the ITS1-5.8S-ITS2 region. The expression analysis of aflR, aflS, aflD, aflP, and aflQ genes in the aflatoxin biosynthesi

SUBMITTER: Okayo RO 

PROVIDER: S-EPMC7492892 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature