Ontology highlight
ABSTRACT:
SUBMITTER: Pareek V
PROVIDER: S-EPMC7494208 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Pareek Vidhi V Tian Hua H Winograd Nicholas N Benkovic Stephen J SJ
Science (New York, N.Y.) 20200401 6488
Metabolons, multiprotein complexes consisting of sequential enzymes of a metabolic pathway, are proposed to be biosynthetic "hotspots" within the cell. However, experimental demonstration of their presence and functions has remained challenging. We used metabolomics and in situ three-dimensional submicrometer chemical imaging of single cells by gas cluster ion beam secondary ion mass spectrometry (GCIB-SIMS) to directly visualize de novo purine biosynthesis by a multienzyme complex, the purinoso ...[more]