Ontology highlight
ABSTRACT:
SUBMITTER: Ho CM
PROVIDER: S-EPMC7494424 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Ho Chi-Min CM Li Xiaorun X Lai Mason M Terwilliger Thomas C TC Beck Josh R JR Wohlschlegel James J Goldberg Daniel E DE Fitzpatrick Anthony W P AWP Zhou Z Hong ZH
Nature methods 20191125 1
X-ray crystallography often requires non-native constructs involving mutations or truncations, and is challenged by membrane proteins and large multicomponent complexes. We present here a bottom-up endogenous structural proteomics approach whereby near-atomic-resolution cryo electron microscopy (cryoEM) maps are reconstructed ab initio from unidentified protein complexes enriched directly from the endogenous cellular milieu, followed by identification and atomic modeling of the proteins. The pro ...[more]