Spark-based parallel calculation of 3D fourier shell correlation for macromolecule structure local resolution estimation.

ABSTRACT:

Background

Resolution estimation is the main evaluation criteria for the reconstruction of macromolecular 3D structure in the field of cryoelectron microscopy (cryo-EM). At present, there are many methods to evaluate the 3D resolution for reconstructed macromolecular structures from Single Particle Analysis (SPA) in cryo-EM and subtomogram averaging (SA) in electron cryotomography (cryo-ET). As global methods, they measure the resolution of the structure as a whole, but they are inaccurate in detecting subtle local changes of reconstruction. In order to detect the subtle changes of reconstruction of SPA and SA, a few local resolution methods are proposed. The mainstream local resolution evaluation methods are based on local Fourier shell correlation (FSC), which is computationally

SUBMITTER: Lu Y 

PROVIDER: S-EPMC7495889 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature