Computationally identifying hot spots in protein-DNA binding interfaces using an ensemble approach.

ABSTRACT:

Background

Protein-DNA interaction governs a large number of cellular processes, and it can be altered by a small fraction of interface residues, i.e., the so-called hot spots, which account for most of the interface binding free energy. Accurate prediction of hot spots is critical to understand the principle of protein-DNA interactions. There are already some computational methods that can accurately and efficiently predict a large number of hot residues. However, the insufficiency of experimentally validated hot-spot residues in protein-DNA complexes and the low diversity of the employed features limit the performance of existing methods.

Results

Here, we report a new computational method for effectively predicting hot spots in protein-DNA binding interfaces. This method,

SUBMITTER: Pan Y 

PROVIDER: S-EPMC7495898 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature