Ontology highlight
ABSTRACT:
SUBMITTER: Nandety RS
PROVIDER: S-EPMC7496502 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
Nandety Raja Sekhar RS Serrani-Yarce Juan C JC Gill Upinder S US Oh Sunhee S Lee Hee-Kyung HK Zhang Xinji X Dai Xinbin X Zhang Wenchao W Krom Nick N Wen Jiangqi J Zhao Patrick X PX Mysore Kirankumar S KS
The Plant journal : for cell and molecular biology 20200623 5
Brachypodium distachyon is an annual C3 grass used as a monocot model system in functional genomics research. Insertional mutagenesis is a powerful tool for both forward and reverse genetics studies. In this study, we explored the possibility of using the tobacco retrotransposon Tnt1 to create a transposon-based insertion mutant population in B. distachyon. We developed transgenic B. distachyon plants expressing Tnt1 (R0) and in the subsequent regenerants (R1) we observed that Tnt1 actively tran ...[more]