Ontology highlight
ABSTRACT:
SUBMITTER: Geyer K
PROVIDER: S-EPMC7496768 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
Geyer Kyra K Sundaram Srividhya S Sušnik Peter P Koert Ulrich U Erb Tobias J TJ
Chembiochem : a European journal of chemical biology 20200330 14
Polyketide synthases (PKSs) use simple extender units to synthesize complex natural products. A fundamental question is how different extender units are site-specifically incorporated into the growing polyketide. Here we established phoslactomycin (Pn) PKS, which incorporates malonyl- and ethylmalonyl-CoA, as an in vitro model to study substrate specificity. We combined up to six Pn PKS modules with different termination sites for the controlled release of tetra-, penta- and hexaketides, and cha ...[more]