Ontology highlight
ABSTRACT:
SUBMITTER: Macpherson A
PROVIDER: S-EPMC7498065 | biostudies-literature | 2020 Sep
REPOSITORIES: biostudies-literature
Macpherson Alex A Scott-Tucker Anthony A Spiliotopoulos Anastasios A Simpson Catherine C Staniforth Justin J Hold Adam A Snowden James J Manning Leah L van den Elsen Jean J Lawson Alastair D G ADG
PLoS biology 20200904 9
As a novel alternative to established surface display or combinatorial chemistry approaches for the discovery of therapeutic peptides, we present a method for the isolation of small, cysteine-rich domains from bovine antibody ultralong complementarity-determining regions (CDRs). We show for the first time that isolated bovine antibody knob domains can function as autonomous entities by binding antigen outside the confines of the antibody scaffold. This yields antibody fragments so small as to be ...[more]