Efficacy of microbicides for inactivation of Ebola-Makona virus on a non-porous surface: a targeted hygiene intervention for reducing virus spread.

ABSTRACT: Microbicides play critical roles in infection prevention and control of Ebola virus by decontaminating high-touch environmental surfaces (HITES), interrupting the virus-HITES-hands nexus. We evaluated the efficacy of formulations containing different microbicidal actives for inactivating Ebola virus-Makona strain (EBOV/Mak) on stainless-steel carriers per ASTM E2197-11. Formulations of sodium hypochlorite (NaOCl) (0.05-1%), ethanol (70%), chloroxylenol (PCMX) (0.12-0.48% by weight) in hard water, and a ready-to-use disinfectant spray with 58% ethanol (EDS), were tested at contact times of 0, or 0.5 to 10 min at ambient temperature. EBOV/Mak was inactivated (>?6 log10) by 70% ethanol after contact times???2.5 min, by 0.5% and 1% NaOCl or EDS (>?4 log10) at contact times???5 min, and by 0.12-0.48% PCMX (>?4.2 log10) at contact times???5 min. Residual infectious virus in neutralized samples was assessed by passage on cells and evaluation for viral cytopathic effect. No infectious virus was detected in cells inoculated with EBOV/Mak exposed to NaOCl (0.5% or 1%), PCMX (0.12% to 0.48%), or EDS for???5 min. These results demonstrate???6 log10 inactivation of EBOV/Mak dried on prototypic surfaces by EDS or formulations of NaOCl (??0.5%), PCMX (??0.12%), or 70% ethanol at contact times???5 min.

SUBMITTER: Cutts TA 

PROVIDER: S-EPMC7498580 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature