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Phycocyanin Extracted from Oscillatoria minima show Antimicrobial, Algicidal, and Antiradical activities: In-silico and In-vitro Analysis.

ABSTRACT: BACKGROUND:Phycocyanin is an algae-derived protein, which binds to pigment for harvesting light. It has been reported in various different species, including that of red algae, dinoflagellates, and cryptophyta. Importantly, phycocyanin has an enormous applications, including cosmetic colorant, food additive, biotechnology, diagnostics, fluorescence detection probe, anticancer agent, anti-inflammatory, immune enhancer, etc. In addition, several different algae were utilized for the isolation of C-PC, but most of the purification methods consist of several steps of crude extraction. AIM:To isolate C-PC from a new source of microalgae with better purity level and to evaluate its antimicrobial, algicidal, and antiradical activities. METHODS:Biological activity, permeability, pharmacokinetics, and toxicity profile of C-PC was predicted by in-silico studies. C-PC was purified and isolated by using ammonium sulphate precipitation, ion-exchange chromatography, gel-filtration chromatography. C-PC was characterized by SDS-PAGE and elution profile (purity ratio) analysis. Antimicrobial and algicial activities of C-PC was evaluated by microtitre plate based assys. Antiradical activity of C-PC was evaluated by DPPH- and ABTS*+ radical scavenging assays. CONCLUSION:C-PC was extracted from Oscillatoria minima for the first time, followed by its quantitative as well qualitative evaluation, indicating a new alternative source of this important protein. Furthermore, the antimicrobial, algicidal, and antiradical activities of the isolated C-PC extract have been demonstrated by both in-silico as well as in-vitro methods.

SUBMITTER: Vaishali CV

PROVIDER: S-EPMC7499352 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature

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Phycocyanin Extracted from Oscillatoria minima Shows Antimicrobial, Algicidal, and Antiradical Activities: In silico and In vitro Analysis.

Venugopal Vaishali C VC Thakur Abhimanyu A Chennabasappa Latha K LK Mishra Gaurav G Singh Kunal K Rathee Parth P Ranjan Anjali A

Anti-inflammatory & anti-allergy agents in medicinal chemistry 20200101 3

<h4>Background</h4>Phycocyanin is an algae-derived protein, which binds to pigment for harvesting light. It has been reported in various different species, including that of red algae, dinoflagellates, and cryptophyta. Importantly, phycocyanin has enormous applications, including cosmetic colorant, food additive, biotechnology, diagnostics, fluorescence detection probe, an anticancer agent, anti-inflammatory, immune enhancer, etc. In addition, several different algae were utilized for the isolat ...[more]

PMID: 30950358

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Project description:BackgroundSeveral studies have been reported previously on the bioactivities of different extracts of marine molluscs. Therefore, we decided to evaluate the cytotoxic and antimicrobial activities of S. pharaonis ink as a highly populated species in the Red Sea. We extracted the flavonoids from the ink and analyzed their composition. Then we evaluated systematically the cytotoxic and antimicrobial properties of this extract. A pharmacokinetic study was also conducted using SwissADME to assess the potential of the identified flavonoids and phenolic compounds from the ink extract to be orally active drug candidates.ResultsCytotoxic activity was evaluated against 5 cell lines (MCF7, Hep G2, A549, and Caco2) at different concentrations (0.4 µg/mL, 1.6 µg/mL, 6.3 µg/mL, 25 µg/mL, 100 µg/mL). The viability of examined cells was reduced by the extract in a concentration-dependent manner. The highest cytotoxic effect of the extract was recorded against A549 and Hep G2 cancer cell lines cells with IC50 = 2.873 and 7.1 µg/mL respectively. The mechanistic analysis by flow cytometry of this extract on cell cycle progression and apoptosis induction indicated that the extract arrests the cell cycle at the S phase in Hep G2 and MCF7, while in A549 cell arrest was recorded at G1 phase. However, it causes G1 and S phase arrest in Caco2 cancer cell line. Our data showed that the extract has significant antimicrobial activity against all tested human microbial pathogens. However, the best inhibitory effect was observed against Candida albicans ATCC 10,221 with a minimum inhibitory concentration (MIC) of 1.95 µg/mL. Pharmacokinetic analysis using SwissADME showed that most flavonoids and phenolics compounds have high drug similarity as they satisfy Lipinski's criteria and have WLOGP values below 5.88 and TPSA below 131.6 Å2.ConclusionS. pharaonis ink ethanolic extract showed a promising cytotoxic potency against various cell lines and a remarkable antimicrobial action against different pathogenic microbial strains. S. pharaonis ink is a novel source of important flavonoids that could be used in the future in different applications as a naturally safe and feasible alternative of synthetic drugs.

Dataset Information

Phycocyanin Extracted from Oscillatoria minima show Antimicrobial, Algicidal, and Antiradical activities: In-silico and In-vitro Analysis.

Publications

Phycocyanin Extracted from Oscillatoria minima Shows Antimicrobial, Algicidal, and Antiradical Activities: In silico and In vitro Analysis.

Similar Datasets

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