Ontology highlight
ABSTRACT:
SUBMITTER: Godoy PM
PROVIDER: S-EPMC7499898 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Godoy Paula M PM Barczak Andrea J AJ DeHoff Peter P Srinivasan Srimeenakshi S Etheridge Alton A Galas David D Das Saumya S Erle David J DJ Laurent Louise C LC
Cell reports 20191201 12
Given the increasing interest in their use as disease biomarkers, the establishment of reproducible, accurate, sensitive, and specific platforms for microRNA (miRNA) quantification in biofluids is of high priority. We compare four platforms for these characteristics: small RNA sequencing (RNA-seq), FirePlex, EdgeSeq, and nCounter. For a pool of synthetic miRNAs, coefficients of variation for technical replicates are lower for EdgeSeq (6.9%) and RNA-seq (8.2%) than for FirePlex (22.4%); nCounter ...[more]