Crocetin Overproduction in Engineered Saccharomyces cerevisiae via Tuning Key Enzymes Coupled With Precursor Engineering.
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ABSTRACT:
SUBMITTER: Song T
PROVIDER: S-EPMC7500066 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
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Crocetin Overproduction in Engineered <i>Saccharomyces cerevisiae</i> via Tuning Key Enzymes Coupled With Precursor Engineering.
Song Tianqing T Wu Nan N Wang Chen C Wang Ying Y Chai Fenghua F Ding Mingzhu M Li Xia X Yao Mingdong M Xiao Wenhai W Yuan Yingjin Y
Frontiers in bioengineering and biotechnology 20200904
Crocetin, an important natural carotenoid dicarboxylic acid with high pharmaceutical values, has been successfully generated from glucose by engineered <i>Saccharomyces cerevisiae</i> in our previous study. Here, a systematic optimization was executed for crocetin overproduction in yeast. The effects of precursor enhancement on crocetin production were investigated by blocking the genes involved in glyoxylate cycle [citric acid synthase (<i>CIT2</i>) and malic acid synthase (<i>MLS1</i>)]. Croce ...[more]
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