Dataset Information

The Molecular Basis and Biologic Significance of the ?-Dystroglycan-Emerin Interaction.

ABSTRACT: ?-dystroglycan (?-DG) assembles with lamins A/C and B1 and emerin at the nuclear envelope (NE) to maintain proper nuclear architecture and function. To provide insight into the nuclear function of ?-DG, we characterized the interaction between ?-DG and emerin at the molecular level. Emerin is a major NE protein that regulates multiple nuclear processes and whose deficiency results in Emery-Dreifuss muscular dystrophy (EDMD). Using truncated variants of ?-DG and emerin, via a series of in vitro and in vivo binding experiments and a tailored computational analysis, we determined that the ?-DG-emerin interaction is mediated at least in part by their respective transmembrane domains (TM). Using surface plasmon resonance assays we showed that emerin binds to ?-DG with high affinity (KD in the nanomolar range). Remarkably, the analysis of cells in which DG was knocked out demonstrated that loss of ?-DG resulted in a decreased emerin stability and impairment of emerin-mediated processes. ?-DG and emerin are reciprocally required for their optimal targeting within the NE, as shown by immunofluorescence, western blotting and immunoprecipitation assays using emerin variants with mutations in the TM domain and B-lymphocytes of a patient with EDMD. In summary, we demonstrated that ?-DG plays a role as an emerin interacting partner modulating its stability and function.

SUBMITTER: Gomez-Monsivais WL

PROVIDER: S-EPMC7504044 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Publications

The Molecular Basis and Biologic Significance of the β-Dystroglycan-Emerin Interaction.

Gómez-Monsivais Wendy Lilián WL Monterrubio-Ledezma Feliciano F Huerta-Cantillo Jazmin J Mondragon-Gonzalez Ricardo R Alamillo-Iniesta Alma A García-Aguirre Ian I Azuara-Medina Paulina Margarita PM Arguello-García Raúl R Rivera-Monroy Jhon Erick JE Holaska James M JM Hernández-Méndez Jesús Mauricio Ernesto JME Garrido Efraín E Magaña Jonathan Javier JJ Winder Steve J SJ Brancaccio Andrea A Martínez-Vieyra Ivette I Navarro-Garcia Fernando F Cisneros Bulmaro B

International journal of molecular sciences 20200819 17

β-dystroglycan (β-DG) assembles with lamins A/C and B1 and emerin at the nuclear envelope (NE) to maintain proper nuclear architecture and function. To provide insight into the nuclear function of β-DG, we characterized the interaction between β-DG and emerin at the molecular level. Emerin is a major NE protein that regulates multiple nuclear processes and whose deficiency results in Emery-Dreifuss muscular dystrophy (EDMD). Using truncated variants of β-DG and emerin, via a series of in vitro a ...[more]

PMID: 32824881

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Dataset Information

The Molecular Basis and Biologic Significance of the ?-Dystroglycan-Emerin Interaction.

Publications

The Molecular Basis and Biologic Significance of the β-Dystroglycan-Emerin Interaction.

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