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Identification and Structural Analysis of Spirostanol Saponin from Yucca schidigera by Integrating Silica Gel Column Chromatography and Liquid Chromatography/Mass Spectrometry Analysis.


ABSTRACT: Yucca schidigera Roezl (Mojave), a kind of ornamental plant belonging to the Yucca genus (Agavaceae), whose extract exhibits important roles in food, beverage, cosmetic and feed additives owing to its rich spirostanol saponins. To provide a comprehensive chemical profiling of the spirostanol saponins in it, this study was performed by using a multi-phase liquid chromatography method combining a reversed phase chromatography T3 column with a normal phase chromatography silica column for the separation and an ESI-Q-Exactive-Orbitrap MS in positive ion mode as the detector. By comparing the retention time and ion fragments with standards, thirty-one spirostanol saponins were identified. In addition, according to the summary of the chromatographic retention behaviors and the MS/MS cleavage patterns and biosynthetic pathway, another seventy-nine spirostanol saponins were speculatively identified, forty ones of which were potentially new ones. Moreover, ten novel spirostanol saponins (three pairs of (25R/S)-spirostanol saponin isomer mixtures) were targeted for isolation to verify the speculation. Then, the comprehensive chemical profiling of spirostanol saponins from Y. schidigera was reported here firstly.

SUBMITTER: Ruan J 

PROVIDER: S-EPMC7504267 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Identification and Structural Analysis of Spirostanol Saponin from <i>Yucca schidigera</i> by Integrating Silica Gel Column Chromatography and Liquid Chromatography/Mass Spectrometry Analysis.

Ruan Jingya J   Qu Lu L   Zhao Wei W   Gao Chang C   Huang Peijian P   Zheng Dandan D   Han Lifeng L   Yu Haiyang H   Zhang Zixin Z   Zhang Yi Y   Wang Tao T  

Molecules (Basel, Switzerland) 20200824 17


<i>Yucca schidigera</i> Roezl (Mojave), a kind of ornamental plant belonging to the <i>Yucca</i> genus (Agavaceae), whose extract exhibits important roles in food, beverage, cosmetic and feed additives owing to its rich spirostanol saponins. To provide a comprehensive chemical profiling of the spirostanol saponins in it, this study was performed by using a multi-phase liquid chromatography method combining a reversed phase chromatography T<sub>3</sub> column with a normal phase chromatography si  ...[more]

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