The endogenous capacity to produce proinflammatory mediators by the ex vivo human perfused lung.

ABSTRACT:

Background

The ex vivo human perfused lung model has enabled optimizing donor lungs for transplantation and delineating mechanisms of lung injury. Perfusate and airspace biomarkers are a proxy of the lung response to experimental conditions. However, there is a lack of studies evaluating biomarker kinetics during perfusion and after exposure to stimuli. In this study, we analyzed the ex vivo-perfused lung response to three key perturbations: exposure to the perfusion circuit, exogenous fresh whole blood, and bacteria.

Results

Ninety-nine lungs rejected for transplantation underwent ex vivo perfusion. One hour after reaching experimental conditions, fresh whole blood was added to the perfusate (n = 55). Two hours after reaching target temperature, Streptococcus pneumoniae was

SUBMITTER: Leligdowicz A 

PROVIDER: S-EPMC7505905 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature