Dataset Information

Root-associated endophytic bacterial community composition and structure of three medicinal licorices and their changes with the growing year.

ABSTRACT: BACKGROUND:The dried roots and rhizomes of medicinal licorices are widely used worldwide as a traditional medicinal herb, which are mainly attributed to a variety of bioactive compounds that can be extracted from licorice root. Endophytes and plants form a symbiotic relationship, which is an important source of host secondary metabolites. RESULTS:In this study, we used high-throughput sequencing technology and high-performance liquid chromatography to explore the composition and structure of the endophytic bacterial community and the content of bioactive compounds (glycyrrhizic acid, liquiritin and total flavonoids) in different species of medicinal licorices (Glycyrrhiza uralensis, Glycyrrhiza glabra, and Glycyrrhiza inflata) and in different planting years (1-3?years). Our results showed that the contents of the bioactive compounds in the roots of medicinal licorices were not affected by the species, but were significantly affected by the main effect growing year (1-3) (P?

SUBMITTER: Dang H

PROVIDER: S-EPMC7507641 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Root-associated endophytic bacterial community composition and structure of three medicinal licorices and their changes with the growing year.

Dang Hanli H Zhang Tao T Li Guifang G Mu Yudi Y Lv Xinhua X Wang Zhongke Z Zhuang Li L

BMC microbiology 20200921 1

<h4>Background</h4>The dried roots and rhizomes of medicinal licorices are widely used worldwide as a traditional medicinal herb, which are mainly attributed to a variety of bioactive compounds that can be extracted from licorice root. Endophytes and plants form a symbiotic relationship, which is an important source of host secondary metabolites.<h4>Results</h4>In this study, we used high-throughput sequencing technology and high-performance liquid chromatography to explore the composition and s ...[more]

PMID: 32957914

Similar Datasets

Project description:The seed microbiota is considered to be the starting point of the accumulation of plant microbiota, which is conducive to the preservation and germination of seeds and the establishment and development of seedlings. Our understanding of the colonization and migration dynamics of microbial taxa during seed development and maturation is still limited. This study used 16S rRNA high-throughput sequencing to investigate the dynamic changes in the composition and diversity of the endophytic bacterial community during maturation of Amorphophallus muelleri seeds. The results showed that as seeds matured (green to red), the Shannon index of their endophytic bacterial community first decreased and then increased, and the ACE and Chao1 indices of the endophytic bacterial community decreased gradually. The Shannon, ACE, and Chao1 indices of the endophytic bacterial community in the seed coat first decreased and then increased. Principal coordinate analysis of the bacterial communities revealed that the seed coat at different maturity stages showed significantly distinct bacterial communities and formed different clusters according to maturity stage. The bacterial communities of green and red seeds showed a clear separation, but they both overlapped with those of yellow seeds, indicating that some core taxa were present throughout seed maturation, but their relative abundance was dynamically changing. As the seeds grew more mature, the relative abundance of some bacterial communities with plant growth-promoting traits and others correlated with plant resistance (e.g., Burkholderia-Caballeronia-Paraburkholderia, Bacillus, Pseudomonas, Bradyrhizobium, Streptomyces) tended to increase and peaked in fully mature seeds and seed coats. The endophytic bacterial community of A. muelleri seeds seems to be driven by the seed maturation state, which can provide a theoretical basis for a comprehensive understanding of the assembly process of the microbial community during A. muelleri seed maturation.

Project description:The root-knot nematode (RKN) is an important pathogen that affects the growth of many crops. Exploring the interaction of biocontrol bacteria-pathogens-host root microbes is the theoretical basis for improving colonization and controlling the effect of biocontrol bacteria in the rhizosphere. Therefore, 16S and 18S rRNA sequencing technology was used to explore the microbial composition and diversity of tobacco roots (rhizosphere and endophytic) at different growth stages in typical tobacco RKN-infected areas for 2 consecutive years. We observed that RKN infection changed the α-diversity and microbial composition of root microorganisms and drove the transformation of microorganisms from bacteria to fungi. The abundance of Sphingomonas decreased significantly from 18% to less than 3%, while the abundance of Rhizobiaceae increased from 4 to 15% at the early growth stage during the first planting year, and it promoted the proliferation of Chryseobacterium at the late growth stage in rhizosphere microorganisms with the highest abundance of 17%. The overall trend of rhizosphere microorganisms changed in the early growth stage with increasing growth time. The specific results were as follows: (1) Rhizobiaceae and Chryseobacterium increased rapidly after 75 days, became the main abundant bacteria in the rhizosphere microorganisms. (2) The dominant flora in fungi were Fusarium and Setophoma. (3) Comparing the root microbes in 2017 and 2018, RKN infection significantly promoted the proliferation of Pseudomonas and Setophoma in both the rhizosphere and endophytes during the second year of continuous tobacco planting, increasing the relative abundance of Pseudomonas from 2 to 25%. Pseudomonas was determined to play an important role in plant pest control. Finally, a total of 32 strains of growth-promoting bacteria were screened from tobacco rhizosphere bacteria infected with RKN through a combination of 16S rRNA sequencing and life-promoting tests. The results of this research are helpful for analyzing the relationship between RKNs and bacteria in plants, providing reference data for elucidating the pathogenesis of RKNs and new ideas for the biological control of RKNs. GRAPHICAL ABSTRACT.

Dataset Information

Root-associated endophytic bacterial community composition and structure of three medicinal licorices and their changes with the growing year.

Publications

Root-associated endophytic bacterial community composition and structure of three medicinal licorices and their changes with the growing year.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets