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Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants.

ABSTRACT: Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable to 10% (v/v) DMSO, and the cells could be differentiated, showing this polymer material can be used to improve, or replace, current cryopreservation strategies.

SUBMITTER: Murray KA

PROVIDER: S-EPMC7509910 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Low DMSO Cryopreservation of Stem Cells Enabled by Macromolecular Cryoprotectants.

Murray Kathryn A KA Tomás Ruben M F RMF Gibson Matthew I MI

ACS applied bio materials 20200818 9

Mesenchymal stromal (stem) cells have potential in regenerative medicine and modulating the immune system. To deliver any cell-based therapy to the patient, it must be cryopreserved, most commonly in DMSO, which impacts cell function and causes clinical side effects. Here we report the use of a synthetically scalable polyampholyte to rescue the cryopreservation of mesenchymal stromal cells in low [DMSO] cryopreservation. Flow cytometry showed retention of key markers of multipotency comparable t ...[more]

PMID: 32984779

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