Dataset Information

The whole genome assembly and evolution analyze of carmine radish (Raphanus sativus L.) Mitochondrion.

ABSTRACT: Carmine radish, which contained a high natural red pigment (red radish pigment), was peculiar produced in Fuling, Chongqing City. Here, the complete nucleotide sequences of the mitochondrial (mt) genome of carmine radish (Raphanus sativus L.) have been determined with a circular sequence with the lengths of 258,965 bp, comprised of 40 protein-coding genes, 23 tRNA genes, and three ribosomal RNA genes. To demonstrate the evolution of organelles genomes in plants, other plant mitochondrial genomes' evolution were also selected for analyze. The results showed that carmine radish (Raphanus sativus L.) is related to MS_Gensuke (Raphanus sativus L.) and Black radish (Raphanus sativus L.), as well as related to Brassica nigra and Brassica carinata, comparing with other Brassicaceae species. This study will provide important genetic tools for other Brassicaceae species research and improve yields of economically important plants.

SUBMITTER: Peng H

PROVIDER: S-EPMC7510604 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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The whole genome assembly and evolution analyze of carmine radish (<i>Raphanus sativus L.</i>) Mitochondrion.

Peng Hua H Gao Jian J

Mitochondrial DNA. Part B, Resources 20200601 3

Carmine radish, which contained a high natural red pigment (red radish pigment), was peculiar produced in Fuling, Chongqing City. Here, the complete nucleotide sequences of the mitochondrial (mt) genome of carmine radish (<i>Raphanus sativus L.</i>) have been determined with a circular sequence with the lengths of 258,965 bp, comprised of 40 protein-coding genes, 23 tRNA genes, and three ribosomal RNA genes. To demonstrate the evolution of organelles genomes in plants, other plant mitochondrial ...[more]

PMID: 33366995

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Project description:Floral induction that initiates bolting and flowering is crucial for reproductive fitness in radishes. CONSTANS-like (CO-like, COL) genes play an important role in the circadian clock, which ensures regular development through complicated time-keeping mechanisms. However, the specific biological and functional roles of each COL transcription factor gene in the radish remain unknown. In this study, we performed a genome-wide identification of COL genes in the radish genome of three cultivars including 'Aokubi', 'kazusa' and 'WK10039', and we analyzed their exon-intron structure, gene phylogeny and synteny, and expression levels in different tissues. The bioinformatics analysis identified 20 COL transcription factors in the radish genome, which were divided into three subgroups (Group I to Group III). RsaCOL-09 and RsaCOL-12 might be tandem duplicated genes, whereas the others may have resulted from segmental duplication. The Ka/Ks ratio indicated that all the COL genes in radish, Arabidopsis, Brassica rapa, Brassica oleracea, Capsella rubella and rice were under purifying selection. We identified 6 orthologous and 19 co-orthologous COL gene pairs between the radish and Arabidopsis, and we constructed an interaction network among these gene pairs. The expression values for each COL gene during vegetable and flower development showed that the majority of Group I members had similar expression patterns. In general, the expression of radish COL genes in Groups I and III decreased during development, whereas the expression of radish COL genes in Group II first increased and then decreased. Substantial numbers of radish COL genes were differentially expressed after vernalization treatment. The expression levels of RsaCOL-02 and RsaCOL-04 were significantly increased during vernalization treatment, while the expression of RsaCOL-10 was significantly decreased. These outcomes provide insights for improving the genetic control of bolting and flowering in radish and other root vegetable crops, and they facilitate genetic improvements to radish yields and quality.

Project description:Carmine radish is famous for containing a natural red pigment (red radish pigment). However, the expression of anthocyanin biosynthesis-related genes during the dynamic development stages of the fleshy roots in carmine radish has not been fully investigated. Here, based on HPLC quantification of anthocyanin levels from our previous study, young fleshy roots of the carmine radish "Hongxin 1" obtained at the dynamic development stages of fleshy roots (seedling stage (SS), initial expansion (IE), full expansion (FE), bolting stage (BS), initial flowering stage (IFS), full bloom stage (FBS) and podding stage (PS)) were used for RNA-Seq. Approximately 126 comodulated DEGs related to anthocyanin biosynthesis (common DEGs in the dynamic growth stages of fleshy roots in carmine radish) were identified, from which most DEGs appeared to be likely to participate in anthocyanin biosynthesis, including two transcription factors, RsMYB and RsRZFP. In addition, some related proteins, e.g., RsCHS, RsDFR, RsANS, RsF'3H, RsF3GGT1, Rs3AT1, RsGSTF12, RsUFGT78D2 and RsUDGT-75C1, were found as candidate contributors to the regulatory mechanism of anthocyanin synthesis in the fleshy roots of carmine radish. In addition, 11 putative DEGs related to anthocyanin synthesis were evaluated by qRT-PCR via the (2-ΔΔCT) method; the Pearson correlation analysis indicated excellent concordance between the RNA-Seq and qRT-PCR results. Furthermore, GO enrichment analysis showed that "anthocyanin-containing compound biosynthetic process" and "anthocyanin-containing compound metabolic process" were commonly overrepresented in the dynamic growth stages of fleshy roots after the initial expansion stage. Moreover, five significantly enriched pathways were identified among the DEGs in the dynamic growth stages of fleshy roots in carmine radish, namely, flavonoid biosynthesis, flavone and flavonol biosynthesis, diterpenoid biosynthesis, anthocyanin biosynthesis, and benzoxazinoid biosynthesis. In conclusion, these results will expand our understanding of the complex molecular mechanisms of anthocyanin biosynthesis in the fleshy roots of carmine radish and the putative candidate genes involved in this process.

Dataset Information

The whole genome assembly and evolution analyze of carmine radish (Raphanus sativus L.) Mitochondrion.

Publications

The whole genome assembly and evolution analyze of carmine radish (<i>Raphanus sativus L.</i>) Mitochondrion.

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