Unknown

Dataset Information

0

Structural analysis of the Legionella pneumophila Dot/Icm type IV secretion system core complex.

ABSTRACT: Legionella pneumophila is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires' Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver effector proteins into host cells. Compared to prototypical T4SSs, the Dot/Icm assembly is much larger, containing ~27 different components including a core complex reported to be composed of five proteins: DotC, DotD, DotF, DotG, and DotH. Using single particle cryo-electron microscopy (cryo-EM), we report reconstructions of the core complex of the Dot/Icm T4SS that includes a symmetry mismatch between distinct structural features of the outer membrane cap (OMC) and periplasmic ring (PR). We present models of known core complex proteins, DotC, DotD, and DotH, and two structurally similar proteins within the core complex, DotK and Lpg0657. This analysis reveals the stoichiometry and contact interfaces between the key proteins of the Dot/Icm T4SS core complex and provides a framework for understanding a complex molecular machine.

SUBMITTER: Durie CL 

PROVIDER: S-EPMC7511231 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Structural analysis of the <i>Legionella pneumophila</i> Dot/Icm type IV secretion system core complex.

Durie Clarissa L CL   Sheedlo Michael J MJ   Chung Jeong Min JM   Byrne Brenda G BG   Su Min M   Knight Thomas T   Swanson Michele M   Lacy D Borden DB   Ohi Melanie D MD  

eLife 20200902

<i>Legionella pneumophila</i> is an opportunistic pathogen that causes the potentially fatal pneumonia Legionnaires' Disease. This infection and subsequent pathology require the Dot/Icm Type IV Secretion System (T4SS) to deliver effector proteins into host cells. Compared to prototypical T4SSs, the Dot/Icm assembly is much larger, containing ~27 different components including a core complex reported to be composed of five proteins: DotC, DotD, DotF, DotG, and DotH. Using single particle cryo-ele  ...[more]

Similar Datasets

Unknown Structural study of Legionella pneumophila effector DotY (Lpg0294), a component of the Dot/Icm type IV secretion system.
| S-EPMC9254896 | biostudies-literature
Unknown Legionella pneumophila strain 130b possesses a unique combination of type IV secretion systems and novel Dot/Icm secretion system effector proteins.
| S-EPMC2976443 | biostudies-literature
Unknown The Legionella pneumophila IcmSW complex interacts with multiple Dot/Icm effectors to facilitate type IV translocation.
| S-EPMC2134951 | biostudies-literature
Unknown In situ structure of the Legionella Dot/Icm type IV secretion system by electron cryotomography.
| S-EPMC5412798 | biostudies-literature
Unknown Identification of the DotL coupling protein subcomplex of the Legionella Dot/Icm type IV secretion system.
| S-EPMC3391322 | biostudies-literature
Unknown DsbA2 (27 kDa Com1-like protein) of Legionella pneumophila catalyses extracytoplasmic disulphide-bond formation in proteins including the Dot/Icm type IV secretion system.
| S-EPMC3086132 | biostudies-literature
Unknown Comprehensive identification of protein substrates of the Dot/Icm type IV transporter of Legionella pneumophila.
| S-EPMC3052360 | biostudies-literature
Unknown Legionella pneumophila DotU and IcmF are required for stability of the Dot/Icm complex.
| S-EPMC517559 | biostudies-literature
Unknown Multiple substrates of the Legionella pneumophila Dot/Icm system identified by interbacterial protein transfer.
| S-EPMC321768 | biostudies-literature
Unknown Poison domains block transit of translocated substrates via the Legionella pneumophila Icm/Dot system.
| S-EPMC3754216 | biostudies-other