Ontology highlight
ABSTRACT:
SUBMITTER: Stein BD
PROVIDER: S-EPMC7512655 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Stein Benjamin D BD Herzig Sébastien S Martínez-Bartolomé Salvador S Lavallée-Adam Mathieu M Shaw Reuben J RJ Yates John R JR
Journal of proteome research 20190906 10
Recent advances in genome editing technologies have enabled the insertion of epitope tags at endogenous loci with relative efficiency. We describe an approach for investigation of protein interaction dynamics of the AMP-activated kinase complex AMPK using a catalytic subunit AMPKα2 (<i>PRKAA2</i> gene) as the bait, based on CRISPR/Cas9-mediated genome editing coupled to stable isotope labeling in cell culture, multidimensional protein identification technology, and computational and statistical ...[more]