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Calprotectin instability may lead to undertreatment in children with IBD.

ABSTRACT: BACKGROUND:Treatment decisions in children with inflammatory bowel disease (IBD) are increasingly based on longitudinal tracking of faecal calprotectin concentrations, but there is little known about the stability of this protein in stool. METHODS:We stored aliquots of homogenised stool at room temperature and at 4°C, and measured the calprotectin concentration for 6 consecutive days with three different assays. In addition, we assessed calprotectin stability in assay-specific extraction buffers kept at room temperature. RESULTS:After 6?days of storage at room temperature, mean percentage change from baseline calprotectin concentrations in stool and extraction buffer was 35% and 46%, respectively. The stability of calprotectin was significantly better preserved in samples stored at 4°C (p=0.0066?and 0.0011, respectively). CONCLUSIONS:Calprotectin is not stable at room temperature. Children with IBD and their caretakers may be falsely reassured by low calprotectin values. The best advisable standard for preanalytical calprotectin handling is refrigeration of the stool sample until delivery at the hospital laboratory.

SUBMITTER: Haisma SM

PROVIDER: S-EPMC7513246 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Calprotectin instability may lead to undertreatment in children with IBD.

Haisma Sjoukje-Marije SM van Rheenen Patrick Ferry PF Wagenmakers Lucie L Muller Kobold Anneke A

Archives of disease in childhood 20190117 10

<h4>Background</h4>Treatment decisions in children with inflammatory bowel disease (IBD) are increasingly based on longitudinal tracking of faecal calprotectin concentrations, but there is little known about the stability of this protein in stool.<h4>Methods</h4>We stored aliquots of homogenised stool at room temperature and at 4°C, and measured the calprotectin concentration for 6 consecutive days with three different assays. In addition, we assessed calprotectin stability in assay-specific ext ...[more]

PMID: 30655264

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Project description:Calprotectin has been well emulated recently in adults as well as in children. The aim of this study was to assess fecal calprotectin concentrations in healthy children aged from 1 to 4 years.Volunteers were enlisted from 3 nurseries. A brief questionnaire was used to ensure these children meet the inclusion criteria, and some clinical and sociodemographic factors were collected. Anthro software (version 3.1) was used to calculated Length-for-age Z-scores (LAZ), weight-for-age Z-scores (WAZ), and weight-for-length Z-scores (WLZ) respectively. Fecal calprotectin was detected by a commercially available ELISA.In total 274 children were recruited, with age ranging from 1 to 4 years old. The median FC concentration was 83.19 ?g/g [range 4.58 to 702.50 ?g/g, interquartile range (IQR) 14.69-419.45 ?g/g] or 1.92 log10 ?g/g (range 0.66 log10 to 2.85 log10 ?g/g, IQR 1.17 log10-2.62 log10 ?g/g). All of the children were divided into three groups, 1-2 years (12-24 months), 2-3 years (24-36 months), 3-4 years (36-48 months), with median FC concentrations 96.14 ?g/g (1.98 log10 ?g/g), 81.48 ?g/g (1.91 log10 ?g/g), 65.36 ?g/g (1.82 log10 ?g/g), respectively. There was similar FC level between boys and girls. FC concentrations showed a downward trend by the growing age groups. A statistic difference was found in FC concentrations among groups 1-2 years, 2-3 years and 3-4 years (P = 0.016). In inter-groups comparison, a significant difference was found between children aged 1-2 years and children aged 3-4 years (P = 0.007). A negative correlation trend was found between age and FC concentration (Spearman's rho = -0.167, P = 0.005) in all the participants. A simple correlation was performed among WLZ, WAZ, birth weight, or birth length with FC, and there was no correlation being observed.Children aged from 1 to 4 years old have lower FC concentrations compared with healthy infants (<1years), and higher FC concentrations when comparing with children older than 4 years and adults.

Dataset Information

Calprotectin instability may lead to undertreatment in children with IBD.

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Calprotectin instability may lead to undertreatment in children with IBD.

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