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Interferon-beta enhances sensitivity to gemcitabine in pancreatic cancer.

ABSTRACT: BACKGROUND:Adjuvant gemcitabine for pancreatic cancer has limited efficacy in the clinical setting. Impaired drug metabolism is associated with treatment resistance. We aimed to evaluate the chemosensitising effect of interferon-beta (IFN-?). METHODS:BxPC-3, CFPAC-1, and Panc-1 cells were pre-treated with IFN-? followed by gemcitabine monotherapy. The effect on cell growth, colony formation, and cell cycle was determined. RT-qPCR was used to measure gene expression. BxPC-3 cells were used in a heterotopic subcutaneous mouse model. RESULTS:IFN-? increased sensitivity to gemcitabine (4-, 7.7-, and 1.7-fold EC50 decrease in BxPC-3, CFPAC-1, and Panc-1, respectively; all P?

SUBMITTER: Blaauboer A

PROVIDER: S-EPMC7513525 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Interferon-beta enhances sensitivity to gemcitabine in pancreatic cancer.

Blaauboer Amber A Booy Stephanie S van Koetsveld Peter M PM Karels Bas B Dogan Fadime F van Zwienen Suzanne S van Eijck Casper H J CHJ Hofland Leo J LJ

BMC cancer 20200923 1

<h4>Background</h4>Adjuvant gemcitabine for pancreatic cancer has limited efficacy in the clinical setting. Impaired drug metabolism is associated with treatment resistance. We aimed to evaluate the chemosensitising effect of interferon-beta (IFN-β).<h4>Methods</h4>BxPC-3, CFPAC-1, and Panc-1 cells were pre-treated with IFN-β followed by gemcitabine monotherapy. The effect on cell growth, colony formation, and cell cycle was determined. RT-qPCR was used to measure gene expression. BxPC-3 cells w ...[more]

PMID: 32967656

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