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HYPERsol: High-Quality Data from Archival FFPE Tissue for Clinical Proteomics.


ABSTRACT: Massive formalin-fixed, paraffin-embedded (FFPE) tissue archives exist worldwide, representing an invaluable resource for clinical proteomics research. However, current protocols for FFPE proteomics lack standardization, efficiency, reproducibility, and scalability. Here we present high-yield protein extraction and recovery by direct solubilization (HYPERsol), an optimized workflow using ultrasonication and S-Trap sample processing that enables proteome coverage and quantification from FFPE samples comparable to that achieved from flash-frozen tissue (average R = 0.936). When applied to archival samples, HYPERsol resulted in high-quality data from FFPE specimens in storage for up to 17 years, and may enable the discovery of new immunohistochemical markers.

SUBMITTER: Marchione DM 

PROVIDER: S-EPMC7515636 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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HYPERsol: High-Quality Data from Archival FFPE Tissue for Clinical Proteomics.

Marchione Dylan M DM   Ilieva Ilyana I   Devins Kyle K   Sharpe Danielle D   Pappin Darryl J DJ   Garcia Benjamin A BA   Wilson John P JP   Wojcik John B JB  

Journal of proteome research 20200114 2


Massive formalin-fixed, paraffin-embedded (FFPE) tissue archives exist worldwide, representing an invaluable resource for clinical proteomics research. However, current protocols for FFPE proteomics lack standardization, efficiency, reproducibility, and scalability. Here we present high-yield protein extraction and recovery by direct solubilization (HYPERsol), an optimized workflow using ultrasonication and S-Trap sample processing that enables proteome coverage and quantification from FFPE samp  ...[more]

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