Ontology highlight
ABSTRACT:
SUBMITTER: Marchione DM
PROVIDER: S-EPMC7515636 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Marchione Dylan M DM Ilieva Ilyana I Devins Kyle K Sharpe Danielle D Pappin Darryl J DJ Garcia Benjamin A BA Wilson John P JP Wojcik John B JB
Journal of proteome research 20200114 2
Massive formalin-fixed, paraffin-embedded (FFPE) tissue archives exist worldwide, representing an invaluable resource for clinical proteomics research. However, current protocols for FFPE proteomics lack standardization, efficiency, reproducibility, and scalability. Here we present high-yield protein extraction and recovery by direct solubilization (HYPERsol), an optimized workflow using ultrasonication and S-Trap sample processing that enables proteome coverage and quantification from FFPE samp ...[more]