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Genome Sequences of Two Microcystis aeruginosa (Chroococcales, Cyanobacteria) Strains from Florida (United States) with Disparate Toxigenic Potentials.

ABSTRACT: Here, we report the draft genomes of two Microcystis aeruginosa strains, i.e., M. aeruginosa BLCC-F108, which was isolated from a toxic bloom in eutrophic waters in Lake Okeechobee (Florida, USA), and M. aeruginosa BLCC-F158, which was isolated from mesotrophic waters in Lake Tohopekaliga (Florida, USA). Genomic analyses show disparate toxin potentials for these two strains.

SUBMITTER: Lefler FW

PROVIDER: S-EPMC7516152 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Genome Sequences of Two Microcystis aeruginosa (Chroococcales, Cyanobacteria) Strains from Florida (United States) with Disparate Toxigenic Potentials.

Lefler Forrest W FW Barbosa Maximiliano M Berthold David E DE Laughinghouse H Dail HD

Microbiology resource announcements 20200924 39

Here, we report the draft genomes of two Microcystis aeruginosa strains, i.e., M. aeruginosa BLCC-F108, which was isolated from a toxic bloom in eutrophic waters in Lake Okeechobee (Florida, USA), and M. aeruginosa BLCC-F158, which was isolated from mesotrophic waters in Lake Tohopekaliga (Florida, USA). Genomic analyses show disparate toxin potentials for these two strains. ...[more]

PMID: 32972941

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Project description:Toxic cyanobacterial blooms are a major contaminant in inland aquatic ecosystems. Furthermore, toxic blooms are carried downstream by rivers and waterways to estuarine and coastal ecosystems. Concerning marine and estuarine animal species, very little is known about how these species are affected by the exposure to freshwater cyanobacteria and cyanotoxins. So far, most of the knowledge has been gathered from freshwater bivalve molluscs. This work aimed to infer the sensitivity of the marine mussel Mytilus galloprovincialis to single as well as mixed toxic cyanobacterial cultures and the underlying molecular responses mediated by toxic cyanobacteria. For this purpose, a mussel exposure experiment was outlined with two toxic cyanobacteria species, Microcystis aeruginosa and Chrysosporum ovalisporum at 1 × 105 cells/mL, resembling a natural cyanobacteria bloom. The estimated amount of toxins produced by M. aeruginosa and C. ovalisporum were respectively 0.023 pg/cell of microcystin-LR (MC-LR) and 7.854 pg/cell of cylindrospermopsin (CYN). After 15 days of exposure to single and mixed cyanobacteria, a depuration phase followed, during which mussels were fed only non-toxic microalga Parachlorella kessleri. The results showed that the marine mussel is able to filter toxic cyanobacteria at a rate equal or higher than the non-toxic microalga P. kessleri. Filtration rates observed after 15 days of feeding toxic microalgae were 1773.04 mL/ind.h (for M. aeruginosa), 2151.83 mL/ind.h (for C. ovalisporum), 1673.29 mL/ind.h (for the mixture of the 2 cyanobacteria) and 2539.25 mL/ind.h (for the non-toxic P. kessleri). Filtering toxic microalgae in combination resulted in the accumulation of 14.17 ng/g dw MC-LR and 92.08 ng/g dw CYN. Other physiological and biochemical endpoints (dry weight, byssus production, total protein and glycogen) measured in this work did not change significantly in the groups exposed to toxic cyanobacteria with regard to control group, suggesting that mussels were not affected with the toxic microalgae. Nevertheless, proteomics revealed changes in metabolism of mussels related to diet, specially evident in those fed on combined cyanobacteria. Changes in metabolic pathways related with protein folding and stabilization, cytoskeleton structure, and gene transcription/translation were observed after exposure and feeding toxic cyanobacteria. These changes occur in vital metabolic processes and may contribute to protect mussels from toxic effects of the toxins MC-LR and CYN.

Project description:BackgroundCyanobacterial blooms are one of the most common stressors encountered by metazoans living in freshwater lentic systems such as lakes and ponds. Blooms reportedly impair fish health, notably through oxygen depletion and production of bioactive compounds including cyanotoxins. However, in the times of the "microbiome revolution", it is surprising that so little is still known regarding the influence of blooms on fish microbiota. In this study, an experimental approach is used to demonstrate that blooms affect fish microbiome composition and functions, as well as the metabolome of holobionts. To this end, the model teleost Oryzias latipes is exposed to simulated Microcystis aeruginosa blooms of various intensities in a microcosm setting, and the response of bacterial gut communities is evaluated in terms of composition and metabolome profiling. Metagenome-encoded functions are compared after 28 days between control individuals and those exposed to highest bloom level.ResultsThe gut bacterial community of O. latipes exhibits marked responses to the presence of M. aeruginosa blooms in a dose-dependent manner. Notably, abundant gut-associated Firmicutes almost disappear, while potential opportunists increase. The holobiont's gut metabolome displays major changes, while functions encoded in the metagenome of bacterial partners are more marginally affected. Bacterial communities tend to return to original composition after the end of the bloom and remain sensitive in case of a second bloom, reflecting a highly reactive gut community.ConclusionGut-associated bacterial communities and holobiont functioning are affected by both short and long exposure to M. aeruginosa, and show evidence of post-bloom resilience. These findings point to the significance of bloom events to fish health and fitness, including survival and reproduction, through microbiome-related effects. In the context of increasingly frequent and intense blooms worldwide, potential outcomes relevant to conservation biology as well as aquaculture warrant further investigation. Video Abstract.

Dataset Information

Genome Sequences of Two Microcystis aeruginosa (Chroococcales, Cyanobacteria) Strains from Florida (United States) with Disparate Toxigenic Potentials.

Publications

Genome Sequences of Two Microcystis aeruginosa (<i>Chroococcales</i>, <i>Cyanobacteria</i>) Strains from Florida (United States) with Disparate Toxigenic Potentials.

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