Gene Expression Profiling Identifies Akt as a Target for Radiosensitization in Gastric Cancer Cells.

ABSTRACT:

Background

Despite the important role of radiotherapy in cancer treatment, a subset of patients responds poorly to treatment majorly due to radioresistance. Particularly the role of radiotherapy has not been established in gastric cancer (GC). Herein, we aimed to identify a radiosensitivity gene signature and to discover relevant targets to enhance radiosensitivity in GC cells.

Methods

An oligonucleotide microarray (containing 22,740 probes) was performed in 12 GC cell lines prior to radiation. A clonogenic assay was performed to evaluate the survival fraction at 2 Gy (SF2) as a surrogate marker for radiosensitivity. Genes differentially expressed (fold change > 6, q-value < 0.025) were identified between radiosensitive and radioresistant cell lines, and quantitative

SUBMITTER: Kim KH 

PROVIDER: S-EPMC7517358 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature