Neutrophil extracellular traps in stored canine red blood cell units.

ABSTRACT: BACKGROUND:Neutrophil extracellular traps (NETs), webs of DNA and citrullinated histones extruded from activated neutrophils cause transfusion-related acute lung injury. Supernatants of stored red blood cell (RBC) units might promote NETosis in neutrophils from the units or from transfusion recipients. HYPOTHESES:(1) NETs form during storage of canine RBC, (2) leukoreduction (LR) before storage of RBC reduces NETosis, and (3) supernatant from stored, nonleukoreduced (NLR) RBC units induces NETosis in healthy canine neutrophils modeling transfusion recipients. ANIMALS:Six healthy purpose-bred research dogs were utilized for blood donation. METHODS:Prospective controlled study. RBC units were collected from each dog, aseptically divided into 2 equal subunits, 1 of which was leukoreduced, and stored for 42?days. Stored units were sampled biweekly for quantification of NET markers citrullinated histone H3 (Western blot) and cell-free DNA (cfDNA) (DNA dye binding). Unit supernatants were applied ex vivo to canine neutrophils and extracellular DNA release representing NETosis was assessed. RESULTS:Markers of NETs increased during RBC storage (cfDNA P?

SUBMITTER: McQuinn ER 

PROVIDER: S-EPMC7517510 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature