The dynamics between in vitro culture and metabolism: embryonic adaptation to environmental changes.

ABSTRACT: Previous studies have discussed the importance of an optimal range of metabolic activity during preimplantation development. To avoid factors than can trigger an undesirable trajectory, it is important to learn how nutrients and metabolites interact to help launching the correct developmental program of the embryo, and how much the in vitro culture system can impair this process. Here, using the bovine model, we describe a factorial experimental design used to investigate the biochemical and molecular signature of embryos in response to different combinations of morphological features-i.e. speed of development-and external stimuli during in vitro culture-i.e. different oxygen tensions and glucose supplementation. Our analyses demonstrate that the embryos present heterogeneous metabolic res

SUBMITTER: de Lima CB 

PROVIDER: S-EPMC7518437 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature