Dataset Information

Molecular insights into the genome dynamics and interactions between core and acquired genomes of Vibrio cholerae.

ABSTRACT: Bacterial species are hosts to horizontally acquired mobile genetic elements (MGEs), which encode virulence, toxin, antimicrobial resistance, and other metabolic functions. The bipartite genome of Vibrio cholerae harbors sporadic and conserved MGEs that contribute in the disease development and survival of the pathogens. For a comprehensive understanding of dynamics of MGEs in the bacterial genome, we engineered the genome of V. cholerae and examined in vitro and in vivo stability of genomic islands (GIs), integrative conjugative elements (ICEs), and prophages. Recombinant vectors carrying the integration module of these GIs, ICE and CTX?, helped us to understand the efficiency of integrations of MGEs in the V. cholerae chromosome. We have deleted more than 250 acquired genes from 6 different loci in the V. cholerae chromosome and showed contribution of CTX prophage in the essentiality of SOS response master regulator LexA, which is otherwise not essential for viability in other bacteria, including Escherichia coli In addition, we observed that the core genome-encoded RecA helps CTX? to bypass V. cholerae immunity and allow it to replicate in the host bacterium in the presence of similar prophage in the chromosome. Finally, our proteomics analysis reveals the importance of MGEs in modulating the levels of cellular proteome. This study engineered the genome of V. cholerae to remove all of the GIs, ICEs, and prophages and revealed important interactions between core and acquired genomes.

SUBMITTER: Pant A

PROVIDER: S-EPMC7519391 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Molecular insights into the genome dynamics and interactions between core and acquired genomes of Vibrio cholerae.

Pant Archana A Bag Satyabrata S Saha Bipasa B Verma Jyoti J Kumar Pawan P Banerjee Sayantan S Kumar Bhoj B Kumar Yashwant Y Desigamani Anbumani A Maiti Suhrid S Maiti Tushar K TK Banerjee Sanjay K SK Bhadra Rupak K RK Koley Hemanta H Koley Hemanta H Dutta Shanta S Nair G Balakrish GB Ramamurthy Thandavarayan T Das Bhabatosh B

Proceedings of the National Academy of Sciences of the United States of America 20200901 38

Bacterial species are hosts to horizontally acquired mobile genetic elements (MGEs), which encode virulence, toxin, antimicrobial resistance, and other metabolic functions. The bipartite genome of Vibrio cholerae harbors sporadic and conserved MGEs that contribute in the disease development and survival of the pathogens. For a comprehensive understanding of dynamics of MGEs in the bacterial genome, we engineered the genome of V. cholerae and examined in vitro and in vivo stability ...[more]

PMID: 32873641

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Dataset Information

Molecular insights into the genome dynamics and interactions between core and acquired genomes of Vibrio cholerae.

Publications

Molecular insights into the genome dynamics and interactions between core and acquired genomes of <i>Vibrio cholerae</i>.

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