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CD8+ T Cells Require ITK-Mediated TCR Signaling for Migration to the Intestine.

ABSTRACT: The Tec kinase IL-2-inducible T cell kinase (ITK) regulates the expression of TCR-induced genes. Itk-/- T cell responses are impaired but not absent. ITK inhibition prevented colitis disease progression and impaired T cell migration to the colon in mice. To examine the function of ITK in T cell migration to the intestine, we examined the number of gut T cells in Itk-/- mice and then evaluated their expression of gut-homing receptors. Combined with in vitro murine T cell stimulation and in vivo migration assay using congenic B6 mice, we demonstrated an essential role for ITK in T cell migration to the intestine in mice. Reconstitution of Itk-/- mouse CD8+ T cells with IFN regulatory factor 4 restored gut-homing properties, providing mechanistic insight into the function of ITK-mediated signaling in CD8+ T cell migration to the intestinal mucosa in mice.

SUBMITTER: Cho HS

PROVIDER: S-EPMC7521019 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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CD8+ T Cells Require ITK-Mediated TCR Signaling for Migration to the Intestine.

Cho Hyoung-Soo HS Ha Soyoung S Shin Hyun Mu HM Reboldi Andrea A Hall Jason A JA Huh Jun R JR Usherwood Edward J EJ Berg Leslie J LJ

ImmunoHorizons 20200207 2

The Tec kinase IL-2-inducible T cell kinase (ITK) regulates the expression of TCR-induced genes. Itk-/- T cell responses are impaired but not absent. ITK inhibition prevented colitis disease progression and impaired T cell migration to the colon in mice. To examine the function of ITK in T cell migration to the intestine, we examined the number of gut T cells in Itk-/- mice and then evaluated their expression of gut-homing receptors. Combined with in vitro mur ...[more]

PMID: 32034085

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