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Functional evidence that Activin/Nodal signaling is required for establishing the dorsal-ventral axis in the annelid Capitella teleta.


ABSTRACT: The TGF-? superfamily comprises two distinct branches: the Activin/Nodal and BMP pathways. During development, signaling by this superfamily regulates a variety of embryological processes, and it has a conserved role in patterning the dorsal-ventral body axis. Recent studies show that BMP signaling establishes the dorsal-ventral axis in some mollusks. However, previous pharmacological inhibition studies in the annelid Capitella teleta, a sister clade to the mollusks, suggests that the dorsal-ventral axis is patterned via Activin/Nodal signaling. Here, we determine the role of both the Activin/Nodal and BMP pathways as they function in Capitella axis patterning. Antisense morpholino oligonucleotides were targeted to Ct-Smad2/3 and Ct-Smad1/5/8, transcription factors specific to the Activin/Nodal and BMP pathways, respectively. Following microinjection of zygotes, resulting morphant larvae were scored for axial anomalies. We demonstrate that the Activin/Nodal pathway of the TGF-? superfamily, but not the BMP pathway, is the primary dorsal-ventral patterning signal in Capitella These results demonstrate variation in the molecular control of axis patterning across spiralians, despite sharing a conserved cleavage program. We suggest that these findings represent an example of developmental system drift.

SUBMITTER: Lanza AR 

PROVIDER: S-EPMC7522025 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Functional evidence that Activin/Nodal signaling is required for establishing the dorsal-ventral axis in the annelid <i>Capitella teleta</i>.

Lanza Alexis R AR   Seaver Elaine C EC  

Development (Cambridge, England) 20200923 18


The TGF-β superfamily comprises two distinct branches: the Activin/Nodal and BMP pathways. During development, signaling by this superfamily regulates a variety of embryological processes, and it has a conserved role in patterning the dorsal-ventral body axis. Recent studies show that BMP signaling establishes the dorsal-ventral axis in some mollusks. However, previous pharmacological inhibition studies in the annelid <i>Capitella teleta</i>, a sister clade to the mollusks, suggests that the dor  ...[more]

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