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Characterization of blood-derived exosomal proteins after exercise.


ABSTRACT: OBJECTIVE:To assess changes in plasma exosome levels and protein content in mice after long-term exercise. METHODS:We subjected 9-month-old adult C57BL/6J mice to daily treadmill running exercise for 4 weeks prior to the isolation of blood-derived exosomes. Exosomal proteins were identified using mass spectrometry. RESULTS:Extracellular bodies were successfully isolated from mouse blood. Protein levels were altered in blood-derived exosomes after chronic treadmill exercise. Levels of the secretagogue secretogranin 2 were markedly elevated in exercise-induced exosomes. CONCLUSION:Our data suggest that levels of secretogranin 2 were increased in mouse exosomes following chronic treadmill exercise. We conclude that exercise increases exocrine secretion of secretogranin 2.

SUBMITTER: Xiang H 

PROVIDER: S-EPMC7522842 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Characterization of blood-derived exosomal proteins after exercise.

Xiang Hongkai H   Chen Shisheng S   Zhou Junhan J   Guo Junxiu J   Zhou Qingfeng Q   Zhou Qishuang Q  

The Journal of international medical research 20200901 9


<h4>Objective</h4>To assess changes in plasma exosome levels and protein content in mice after long-term exercise.<h4>Methods</h4>We subjected 9-month-old adult C57BL/6J mice to daily treadmill running exercise for 4 weeks prior to the isolation of blood-derived exosomes. Exosomal proteins were identified using mass spectrometry.<h4>Results</h4>Extracellular bodies were successfully isolated from mouse blood. Protein levels were altered in blood-derived exosomes after chronic treadmill exercise.  ...[more]

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