Antibiotic Substrate Selectivity of Pseudomonas aeruginosa MexY and MexB Efflux Systems Is Determined by a Goldilocks Affinity.
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ABSTRACT:
SUBMITTER: Dey D
PROVIDER: S-EPMC7526836 | biostudies-literature | 2020 Jul
REPOSITORIES: biostudies-literature
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Antibiotic Substrate Selectivity of Pseudomonas aeruginosa MexY and MexB Efflux Systems Is Determined by a Goldilocks Affinity.
Dey Debayan D Kavanaugh Logan G LG Conn Graeme L GL
Antimicrobial agents and chemotherapy 20200722 8
Resistance-nodulation-division (RND) efflux pumps are important contributors to bacterial antibiotic resistance. In this study, we combined evolutionary sequence analyses, computational structural modeling, and ligand docking to develop a framework that can explain the known antibiotic substrate selectivity differences between two <i>Pseudomonas aeruginosa</i> RND transporters, MexY and MexB. For efficient efflux, antibiotic substrates must possess a "Goldilocks affinity": binding strong enough ...[more]
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