TCP1? Subunit Is Indispensable for Growth and Infectivity of Leishmania donovani.

ABSTRACT: T-complex protein-1 (TCP1) is a ubiquitous group II chaperonin and is known to fold various proteins, such as actin and tubulin. In Leishmania donovani, the ? subunit of TCP1 (LdTCP1?) has been cloned and characterized. It forms a high-molecular-weight homo-oligomeric complex that performs ATP-dependent protein folding. In the present study, we evaluated the essentiality of the LdTCP1? gene. Gene replacement studies indicated that LdTCP1? is essential for parasite survival. The LdTCP1? single-allele-replacement mutants exhibited slowed growth and decreased infectivity in mouse macrophages compared to the growth and infectivity of the wild-type parasites. Modulation of LdTCP1? expression in promastigotes also modulated cell cycle progression. Suramin, an antitrypanosomal drug, not only inhibited the luciferase refolding activity of the recombinant LdTCP1? (rLdTCP1?) homo-oligomeric complex but also exhibited potential antileishmanial efficacy both in vitro and in vivo The interaction of suramin and LdTCP1? was further validated by isothermal titration calorimetry. The study suggests LdTCP1? as a potential drug target and also provides a framework for the development of a new class of drugs.

SUBMITTER: Yadav S 

PROVIDER: S-EPMC7526847 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature