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Reunification of a split sciatic nerve.

ABSTRACT: Sciatic nerve (SN) variaitons can result in interesting clinical presentations. We identified a SN variant that does not fit into preexisting classification schemes. In an adult male cadaver, the SN was found to divide proximally and partly exit through the piriformis muscle. Distal to the piriformis, the two parts of the SN were reunited. Although apparently extremely rare, such a finding should be added to the archives of anatomical variations.

SUBMITTER: Huang B

PROVIDER: S-EPMC7527125 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Reunification of a split sciatic nerve.

Huang Brendan B Decater Tess T Iwanaga Joe J Dumont Aaron S AS Tubbs R Shane RS

Anatomy & cell biology 20200901 3

Sciatic nerve (SN) variaitons can result in interesting clinical presentations. We identified a SN variant that does not fit into preexisting classification schemes. In an adult male cadaver, the SN was found to divide proximally and partly exit through the piriformis muscle. Distal to the piriformis, the two parts of the SN were reunited. Although apparently extremely rare, such a finding should be added to the archives of anatomical variations. ...[more]

PMID: 32879057

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Project description:Long-range peripheral nerve defect is a severe and worldwide disease. With the increasing development of tissue engineering, the excellent ability of nerve extracellular matrix (ECM) in peripheral nerve injury (PNI) has been widely studied and verified. Here, we present a novel microtube that contains gradient decellularized porcine sciatic nerve ECM hydrogel (pDScNM-gel) from microfluidics for sciatic nerve regeneration. The pDScNM is confirmed to enhance cell proliferation and migration, and improve the axon growth of primary dorsal root ganglions (DRGs) in a concentration-related manner. These behaviors were also achieved when cells were co-cultured in a gradient pDScNM microtube. The in vivo sciatic nerve regeneration and functional recovery were also demonstrated by assembling the gradient pDScNM microtubes with a medical silicon tube. These results indicated that the microtubes with gradient pDScNM could act as a promising alternative for repairing peripheral nerve defects and showed great potential in clinical use.

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Characteristics of cytokines in the sciatic nerve stumps and DRGs after rat sciatic nerve crush injury.

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Project description:BackgroundPeripheral nerves can self-regenerate after traumatic injury, although their self-regeneration ability is limited after severe nerve injury. After peripheral nerve injury, the distal nerve stumps undergo Wallerian degeneration while the proximal nerve stumps undergo a regeneration process.MethodsHere, to decipher genetic changes and involved biological processes in the proximal nerve stumps after peripheral nerve injury, microarray data (GSE30165) were analyzed. Differentially expressed genes in the proximal nerve stumps at 0.5 h, 1 h, 3 h, 6 h, 9 h, 1 d, 4 d, 7 d, and 14 d after rat sciatic nerve transection were subjected to Ingenuity pathway analysis (IPA) bioinformatic analysis.ResultsCytokine signaling, cellular immune response, nuclear receptor signaling, disease-specific pathways, and organismal growth and development were significantly activated in the proximal nerve stumps after nerve transection. Organ development, inflammation and immune response, diseases and organ abnormalities, and cellular behavior-related biological functions were highly involved.ConclusionsThe expression levels of differentially expressed genes in biological function "Organismal Injury and Abnormalities" were displayed and validated. Our current study helps to obtain a better understanding of the biological processes of peripheral nerve regeneration, especially the regeneration process in the proximal nerve stumps, and thus may help to discover new therapeutic methods that can promote nerve regeneration.

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