Dataset Information

Performance of two bone substitutes of novel cotton-like ?-TCP/PDLGA and granular ?-TCP on bone regeneration in the femoral bone defect of the Beagle dogs.

ABSTRACT: This study aimed to clarify whether novel cotton-like composite made of ?-tricalcium phosphate (?-TCP) and poly(Dl-lactide-co-glycolide) (PDLGA) has a different effect on in vivo bone regeneration after bone defect than that of granular ?-TCP. Five male Beagle dogs served as subjects. Cortical and medullary bone defect as non-through holes were made at the diaphysis of the bilateral femurs. One side was implanted with ?-TCP/PDLGA (?-TCP/PDLGA group) and the other side was implanted with granular ?-TCP (?-TCP group). At 4 weeks after implantation, we found no significant differences in the percentages of newly formed bone area and fibrous tissue area in the bone defect between the two groups. The ?-TCP/PDLGA group showed more uniform filling on the surface and earlier disappearance of the material in the medullary region, and there were fewer inflammatory cells and osteoclasts in the bone defect in the ?-TCP/PDLGA group. In conclusion, ?-TCP/PDLGA performs better at filling the bone defect uniformly and disappears earlier at the cortical and medullary regions while causing less inflammation and bone resorption. Although bone formation activity of the ?-TCP/PDLGA group in the cortical region was lower, the newly formed bone volume in bone defect of the ?-TCP/PDLGA group was equal to that of the ?-TCP group.

SUBMITTER: Okada Y

PROVIDER: S-EPMC7528068 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Performance of two bone substitutes of novel cotton-like β-TCP/PDLGA and granular β-TCP on bone regeneration in the femoral bone defect of the Beagle dogs.

Okada Yasuaki Y Yamanaka Yoshiaki Y Menuki Kunitaka K Zenke Yukichi Y Tsukamoto Manabu M Tajima Takafumi T Kosugi Kenji K Kawasaki Makoto M Nakamura Eiichiro E Toyota Naoka N Kawabe Yasuhiro Y Sakai Akinori A

Bone reports 20200924

This study aimed to clarify whether novel cotton-like composite made of β-tricalcium phosphate (β-TCP) and poly(Dl-lactide-<i>co</i>-glycolide) (PDLGA) has a different effect on <i>in vivo</i> bone regeneration after bone defect than that of granular β-TCP. Five male Beagle dogs served as subjects. Cortical and medullary bone defect as non-through holes were made at the diaphysis of the bilateral femurs. One side was implanted with β-TCP/PDLGA (β-TCP/PDLGA group) and the other side was implanted ...[more]

PMID: 33024798

Similar Datasets

Project description:Background: Grafting of biomaterial in alveolar defect facilitates bone healing and orthodontic treatment. BMP2-functionalized biomimetic calcium phosphate (BioCaP) graft had shown excellent bone defect healing potential in many preclinical studies. In this study, we aimed to investigate the influence of BioCaP graft on surgical alveolar bone defect healing during orthodontic tooth movement (OTM) in beagle dogs. Methods: Nine Beagle dogs were randomly assigned to three groups: control, deproteinized bovine bone (DBB), and BioCaP. The maxillary second premolars were protracted into the defects of the extracted maxillary first premolar for 8 weeks. The rate of OTM, alveolar remodeling and bone defect healing were evaluated by histology, histomorphometry, and cone beam computed tomography (CBCT) imaging. Periodontal probing depth was analyzed. Gingival cervicular fluid was collected at week 4 and 8, and the IL-1? level was measured by ELISA. Results: The histological sections of the bone defect showed more newly formed bone in the BioCaP group. The percentage of new bone formation in the BioCaP group was 1.61-, and 1.25-fold higher compared to the control and DBB group, respectively. After 8 weeks of OTM, the resorption rate of BioCaP was 1.42-fold higher compared to DBB. The root resorption index in the DBB group was 1.87-, and 1.39-fold higher compared to the control and BioCaP group, respectively. CBCT images showed 1.92-, and 1.36-fold higher bone mineral density in the BioCaP group compared to the control and DBB group, respectively. There was no significant difference in OTM among the three groups. The distance between the enamel cementum and the crest of the alveolar ridge in the control group was 1.45-, and 1.69-fold higher compared to DBB and BioCaP group, respectively. Periodontal probing depth at week 8 was reduced in the BioCaP group compared to the control. IL-1? concentration in the gingival cervicular fluid was significantly lower in the BioCaP group compared to the control group at week 4 and 8. Conclusion: BioCaP graft robustly promoted bone regeneration and alveolar bone defect healing without affecting OTM. BioCaP graft caused less alveolar bone recession and root resorption of traction tooth with favorable periodontal attachment level indicating that BioCaP as a bioactive and functional bone filling material for alveolar bone defects during orthodontic treatment.

Dataset Information

Performance of two bone substitutes of novel cotton-like ?-TCP/PDLGA and granular ?-TCP on bone regeneration in the femoral bone defect of the Beagle dogs.

Publications

Performance of two bone substitutes of novel cotton-like β-TCP/PDLGA and granular β-TCP on bone regeneration in the femoral bone defect of the Beagle dogs.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets