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Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy.

ABSTRACT: SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Here, we investigated the interaction of this new coronavirus with Vero cells using high resolution scanning electron microscopy. Surface morphology, the interior of infected cells and the distribution of viral particles in both environments were observed 2 and 48 h after infection. We showed areas of viral processing, details of vacuole contents, and viral interactions with the cell surface. Intercellular connections were also approached, and viral particles were adhered to these extensions suggesting direct cell-to-cell transmission of SARS-CoV-2.

SUBMITTER: Caldas LA

PROVIDER: S-EPMC7528159 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature

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Ultrastructural analysis of SARS-CoV-2 interactions with the host cell via high resolution scanning electron microscopy.

Caldas Lucio Ayres LA Carneiro Fabiana Avila FA Higa Luiza Mendonça LM Monteiro Fábio Luiz FL da Silva Gustavo Peixoto GP da Costa Luciana Jesus LJ Durigon Edison Luiz EL Tanuri Amilcar A de Souza Wanderley W

Scientific reports 20200930 1

SARS-CoV-2 is the cause of the ongoing COVID-19 pandemic. Here, we investigated the interaction of this new coronavirus with Vero cells using high resolution scanning electron microscopy. Surface morphology, the interior of infected cells and the distribution of viral particles in both environments were observed 2 and 48 h after infection. We showed areas of viral processing, details of vacuole contents, and viral interactions with the cell surface. Intercellular connections were also approached ...[more]

PMID: 32999356

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