Ontology highlight
ABSTRACT:
SUBMITTER: Hermon K
PROVIDER: S-EPMC7529757 | biostudies-literature | 2020 Oct
REPOSITORIES: biostudies-literature
Hermon Kobi K Schidorsky Shachar S Razvag Yair Y Yakovian Oren O Sherman Eilon E
Scientific reports 20201001 1
Single-molecule-localization-microscopy (SMLM) enables superresolution imaging of biological samples down to ~ 10-20 nm and in single molecule detail. However, common SMLM reconstruction largely disregards information embedded in the entire intensity trajectories of individual emitters. Here, we develop and demonstrate an approach, termed time-correlated-SMLM (tcSMLM), that uses such information for enhancing SMLM reconstruction. Specifically, tcSMLM is shown to increase the spatial resolution a ...[more]