Unknown

Dataset Information

0

Biophysical and biochemical characterization of a VHH-based IgG-like bi- and trispecific antibody platform.


ABSTRACT: Here, we report the characterization of a VHH-derived IgG-like bi- and trispecific antibody platform that essentially relies on the replacement of the VH and VL regions of a conventional antibody by two independently functioning VHH domains. Consequently, a VHH is engrafted onto constant region CH1 while the other VHH-based paratope is engrafted on the constant region of the light chain, Cκ or Cλ, resulting in a tetravalent bispecific IgG-like molecule. Combined with a heavy chain heterodimerization technique, this platform allows facile engineering of bi- and trispecific antibodies with flexible valencies. We demonstrate the general applicability of this generic platform approach and elaborate on the limitations of specific formats.

SUBMITTER: Pekar L 

PROVIDER: S-EPMC7531565 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC8905027 | biostudies-literature
| S-EPMC9874498 | biostudies-literature
| S-EPMC5153528 | biostudies-literature
| S-EPMC6458986 | biostudies-literature
| S-EPMC8557579 | biostudies-literature
| S-EPMC11201533 | biostudies-literature
| S-EPMC10858392 | biostudies-literature
| S-EPMC10373336 | biostudies-literature
| S-EPMC5507608 | biostudies-literature
| S-EPMC6311853 | biostudies-literature