The differences of regulatory networks between papillary and anaplastic thyroid carcinoma: an integrative transcriptomics study.

ABSTRACT:

Background

Unlike papillary thyroid cancer (PTC), anaplastic thyroid carcinoma (ATC) is extremely aggressive and rapidly lethal without effective therapies. However, the differences of master regulators and regulatory networks between PTC and ATC remain unclear. Methods: Three representative datasets comprising 32 ATC, 69 PTC, and 78 normal thyroid tissue samples were combined to form a large dataset. Differentially expressed genes (DEGs) were identified and enriched by limma package and gene set enrichment analysis, respectively. Subsequently, protein-protein interaction network and transcription factors (TFs) regulatory network were constructed to identify gene modules and master regulators. Further, master regulators were validated by RT-PCR and western blot. Finally, Kap

SUBMITTER: Pan Z 

PROVIDER: S-EPMC7537793 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature