Ontology highlight
ABSTRACT:
SUBMITTER: Wen W
PROVIDER: S-EPMC7541815 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Wen Wen W Wang Yongchao Y Li Hui H Xu Hong H Xu Mei M Frank Jacqueline A JA Ma Murong M Luo Jia J
Frontiers in molecular neuroscience 20200924
Neurite outgrowth is essential for brain development and the recovery of brain injury and neurodegenerative diseases. In this study, we examined the role of the neurotrophic factor MANF in regulating neurite outgrowth. We generated MANF knockout (KO) neuro2a (N2a) cell lines using clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 and demonstrated that MANF KO N2a cells failed to grow neurites in response to RA stimulation. Using MANF siRNA, this finding was confirmed in hum ...[more]