MiR-27a Facilitates Breast Cancer Progression via GSK-3?.

ABSTRACT: Breast cancer remains one of the leading causes of cancer-associated death in women. MiR-27a is highly expressed in breast cancer tissue. However, the underlying mechanisms that promote breast cancer progression are unknown. In this study, we investigated the regulatory mechanisms of miR-27a and its target glycogen Synthase Kinase 3-? (GSK-3?) in breast cancer cells. We found that miR-27a was highly expressed in breast cancer tissues, which downregulated GSK-3? expression. We further identified GSK-3? as a direct target of miR-27a, and found that the miR-27a mediated suppression of GSK-3? activated Wnt/?-catenin-associated proliferative and invasive factor in breast cancer. The cell transfection assay demonstrated the overexpression of miR-27a also enhanced cell proliferation and invasion, and reduced cell apoptosis through GSK-3?. Finally, we demonstrated that the overexpression of miR-27a facilitated breast cancer progression through its ability to down-regulate the phosphorylation of GSK-3? both in vivo and vitro. These findings highlighted miR-27a as a novel therapeutic target in breast cancer.

SUBMITTER: Chen H 

PROVIDER: S-EPMC7545786 | biostudies-literature | 2020 Jan-Dec

REPOSITORIES: biostudies-literature