Ontology highlight
ABSTRACT:
SUBMITTER: Jiang W
PROVIDER: S-EPMC7545986 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Jiang Wang W Li Hao H Liu Xiyu X Zhang Jianping J Zhang Wuhu W Li Tianjiao T Liu Liang L Yu Xianjun X
Theranostics 20200916 25
<b>Rationale:</b> Pancreatic ductal adenocarcinoma (PDAC) is a highly lethal disease with few therapeutic targets and rare effective treatments. Over 90% of PDAC tumors bear a Kras mutation, and the single-site mutation G12D (Kras<sup>G12D</sup>) is most prevalent. <b>Methods:</b> Here, we applied the CRISPR-CasRx system to silence the mutant Kras<sup>G12D</sup> transcript in PDAC cells. We also used a capsid-optimized adenovirus-associated virus 8 vector (AAV8) to deliver the CRISPR-CasRx syste ...[more]