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Diversity of rotavirus genotypes circulating in children


ABSTRACT: BACKGROUND:From 2016, the Government of India introduced the oral rotavirus vaccine into the national immunization schedule. Currently, two indigenously developed vaccines (ROTAVAC, Bharat Biotech; ROTASIIL, Serum Institute of India) are included in the Indian immunization program. We report the rotavirus disease burden and the diversity of rotavirus genotypes from 2005 to 2016 in a multi-centric surveillance study before the introduction of vaccines. METHODS:A total of 29,561 stool samples collected from 2005 to 2016 (7 sites during 2005-2009, 3 sites from 2009 to 2012, and 28 sites during 2012-2016) were included in the analysis. Stools were tested for rotavirus antigen using enzyme immunoassay (EIA). Genotyping was performed on 65.8% of the EIA positive samples using reverse transcription- polymerase chain reaction (RT-PCR) to identify the G (VP7) and P (VP4) types. Multinomial logistic regression was used to quantify the odds of detecting genotypes across the surveillance period and in particular age groups. RESULTS:Of the 29,561 samples tested, 10,959 (37.1%) were positive for rotavirus. There was a peak in rotavirus positivity during December to February across all sites. Of the 7215 genotyped samples, G1P[8] (38.7%) was the most common, followed by G2P[4] (12.3%), G9P[4] (5.8%), G12P[6] (4.2%), G9P[8] (4%), and G12P[8] (2.4%). Globally, G9P[4] and G12P[6] are less common genotypes, although these genotypes have been reported from India and few other countries. There was a variation in the geographic and temporal distribution of genotypes, and the emergence or re-emergence of new genotypes such as G3P[8] was seen. Over the surveillance period, there was a decline in the proportion of G2P[4], and an increase in the proportion of G9P[4]. A higher proportion of mixed and partially typed/untyped samples was also seen more in the age group 0-11?months. CONCLUSIONS:This 11?years surveillance highlights the high burden of severe rotavirus gastroenteritis in Indian children

SUBMITTER: Giri S 

PROVIDER: S-EPMC7547507 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Diversity of rotavirus genotypes circulating in children < 5 years of age hospitalized for acute gastroenteritis in India from 2005 to 2016: analysis of temporal and regional genotype variation.

Giri Sidhartha S   Kumar C P Girish CPG   Khakha Shainey Alokit SA   Chawla-Sarkar Mamta M   Gopalkrishna Varanasi V   Chitambar Shobha D SD   Ray Pratima P   Venkatasubramanian S S   Borkakoty Biswa Jyoti BJ   Roy Subarna S   Bhat Jyothi J   Dwibedi Bhagirathi B   Das Pradeep P   Paluru Vijayachari V   Ramani Sasirekha S   Babji Sudhir S   Arora Rashmi R   Mehendale Sanjay M SM   Gupte Mohan D MD   Kang Gagandeep G  

BMC infectious diseases 20201009 1


<h4>Background</h4>From 2016, the Government of India introduced the oral rotavirus vaccine into the national immunization schedule. Currently, two indigenously developed vaccines (ROTAVAC, Bharat Biotech; ROTASIIL, Serum Institute of India) are included in the Indian immunization program. We report the rotavirus disease burden and the diversity of rotavirus genotypes from 2005 to 2016 in a multi-centric surveillance study before the introduction of vaccines.<h4>Methods</h4>A total of 29,561 sto  ...[more]

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