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Automated in-process characterization and selection of cell-clones for quality and efficient cell manufacturing.

ABSTRACT: Delivery of safe, effective and reliable cellular therapies, whether based on mesenchymal stromal cells (MSCs) or induced pluripotent stem cells (iPSCs), demand standardization of cell culture protocols. There is a need to develop automation platform that enables the users to generate culture expanded human cell populations that improves the quality and reduces batch-to-batch variation with respect to biological potential. Cell X™ robot was designed to address these current challenges in the cell fabrication industry. It utilizes non-invasive large field of view quantitative image analysis to guide an automated process of targeted "biopsy" (cells or media), "picking" (selection) of desired cells or colonies, or "weeding" (removal) of undesired cells, thus providing an unprecedented ability to acquire quantitative measurement in a complex heterogeneous cell environment "in process" and then to act on those measurements to define highly reproducible methods for cell and colony "management" based on application specific critical quality attributes to improve the quality of the manufactured cell lines and cell products.

SUBMITTER: Mantripragada VP

PROVIDER: S-EPMC7547927 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Advances in clinical NK cell studies: Donor selection, manufacturing and quality control.

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Additive manufacturing process selection for automotive industry using Pythagorean fuzzy CRITIC EDAS.

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Lentivirus Manufacturing Process for Primary T-Cell Biofactory Production.

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A high-density microfluidic bioreactor for the automated manufacturing of CAR T cells

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2024-05-29 | GSE261103 | GEO

Australia's continental-scale acoustic tracking database and its automated quality control process.

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| S-EPMC5789868 | biostudies-literature

Semi-automated optimized method to isolate CRISPR/Cas9 edited human pluripotent stem cell clones.

Project description:BackgroundCRISPR/Cas9 editing systems are currently used to generate mutations in a particular gene to mimic a genetic disorder in vitro. Such "disease in a dish" models based on human pluripotent stem cells (hPSCs) offer the opportunity to have access to virtually all cell types of the human body. However, the generation of mutated hPSCs remains fastidious. Current CRISPR/Cas9 editing approaches lead to a mixed cell population containing simultaneously non-edited and a variety of edited cells. These edited hPSCs need therefore to be isolated through manual dilution cloning, which is time-consuming, labor intensive and tedious.MethodsFollowing CRISPR/Cas9 edition, we obtained a mixed cell population with various edited cells. We then used a semi-automated robotic platform to isolate single cell-derived clones.ResultsWe optimized CRISPR/Cas9 editing to knock out a representative gene and developed a semi-automated method for the clonal isolation of edited hPSCs. This method is faster and more reliable than current manual approaches.ConclusionsThis novel method of hPSC clonal isolation will greatly improve and upscale the generation of edited hPSCs required for downstream applications including disease modeling and drug screening.

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