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Extended and Fully Automated Newborn Screening Method for Mass Spectrometry Detection.

ABSTRACT: A new and fully automated newborn screening method for mass spectrometry was introduced in this paper. Pathological relevant amino acids, acylcarnitines, and certain steroids are detected within 4 min per sample. Each sample is treated in an automated and standardized workflow, where a mixture of deuterated internal standards is sprayed onto the sample before extraction. All compounds showed good linearity, and intra- and inter-day variation lies within the acceptance criteria (except for aspartic acid). The described workflow decreases analysis cost and labor while improving the sample traceability towards good laboratory practice.

SUBMITTER: Gaugler S

PROVIDER: S-EPMC7548895 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature

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Extended and Fully Automated Newborn Screening Method for Mass Spectrometry Detection.

Gaugler Stefan S Rykl Jana J Wegner Irene I von Däniken Tamara T Fingerhut Ralph R Schlotterbeck Götz G

International journal of neonatal screening 20171229 1

A new and fully automated newborn screening method for mass spectrometry was introduced in this paper. Pathological relevant amino acids, acylcarnitines, and certain steroids are detected within 4 min per sample. Each sample is treated in an automated and standardized workflow, where a mixture of deuterated internal standards is sprayed onto the sample before extraction. All compounds showed good linearity, and intra- and inter-day variation lies within the acceptance criteria (except for aspart ...[more]

PMID: 33072928

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