Dataset Information

Long non?coding RNA RP11?400N13.3 promotes the progression of colorectal cancer by regulating the miR?4722?3p/P2RY8 axis.

ABSTRACT: Accumulating evidence has shown that long non?coding RNAs (lncRNAs) play significant roles in the development and progression of many types of cancer including colorectal cancer. RP11?400N13.3 is a novel lncRNA discovered recently and its biological function and underlying mechanism in colorectal cancer remain elusive. This study aimed to reveal the relationship between RP11?400N13.3 and colorectal cancer. Our results demonstrated that the expression of RP11?400N13.3 was significantly upregulated in both colorectal cancer tissues and cell lines as compared to normal adjacent tissues and normal colonic epithelial cells by RT?qPCR, respectively. Upregulation of RP11?400N13.3 was found to be correlated with a poor overall survival rate. Functional studies revealed that RP11?400N13.3 facilitated the proliferation, migration, invasion and tumor growth of colorectal cancer cells while inhibiting the apoptosis of cancer cells in vitro and in vivo. We also observed that RP11?400N13.3 serves as a sponge for miR?4722?3p, and that P2Y receptor family member 8 (P2RY8) was predicted to be a target of miR?4722?3p by bioinformatics analysis. Western blot assay indicated that the expression of P2RY8 was negatively or positively regulated by miR?4722?3p or RP11?400N13.3. In addition, rescue experiments revealed that RP11?400N13.3 promoted proliferation, migration and invasion by directly regulating the expression of miR?4722?3p and P2RY8. In conclusion, our results revealed that RP11?400N13.3 promoted colorectal cancer progression via modulating the miR?4722?3p/P2RY8 axis, thus suggesting RP11?400N13.3 as a potential therapeutic target for the treatment of colorectal cancer.

SUBMITTER: Yang H

PROVIDER: S-EPMC7551293 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature

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Long non‑coding RNA RP11‑400N13.3 promotes the progression of colorectal cancer by regulating the miR‑4722‑3p/P2RY8 axis.

Yang Hongju H Li Qian Q Wu Yanrui Y Dong Jianlong J Lao Yaling Y Ding Zheng Z Xiao Changyan C Fu Jinxiao J Bai Song S

Oncology reports 20200907 5

Accumulating evidence has shown that long non‑coding RNAs (lncRNAs) play significant roles in the development and progression of many types of cancer including colorectal cancer. RP11‑400N13.3 is a novel lncRNA discovered recently and its biological function and underlying mechanism in colorectal cancer remain elusive. This study aimed to reveal the relationship between RP11‑400N13.3 and colorectal cancer. Our results demonstrated that the expression of RP11‑400N13.3 was significantly upregulate ...[more]

PMID: 32901883

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Project description:Ovarian cancer (OC), the third common gynecologic malignancy, contributes to the most cancer-caused mortality in women. However, 70% of patients with OC are diagnosed at an advanced stage, of which the 5-year survival is less than 30%. Long noncoding RNAs (long ncRNAs or lncRNA), a type of RNA with exceeding 200 nucleotides in length but no protein-coding capability, have been demonstrated to involve the pathogenesis of various cancers and show considerable potential in the diagnosis of OC. In this study, we found that the LINC00909 expression in tumor and serum specimens of OC patients was elevated, determined by real-time quantitative, and droplet digital PCR. In receiver operating characteristic (ROC) analysis, our results revealed that serum LINC00909 distinguished cancers from normal ovarian tissue with 87.8% of sensitivity and 69.6% of specificity (AUC, 81.2%) and distinguished serous ovarian cancer from normal ovarian tissue with 90.0% of sensitivity and 75.9% of specificity (AUC, 84.5%). Furthermore, we observed that the tumor and serum LINC00909 level was positively associated with the International Federation of Gynecology and Obstetrics (FIGO) stage and the Eastern Cooperative Oncology Group (ECOG) score (reflecting patients' performance status). Also, patients with low serum LINC00909 level showed a longer overall (hazard ratio, HR = 1.874, p = 0.0004) and progression-free (HR = 1.656, p = 0.0017) survival. Functional assays indicated that the elevation of LINC00909 expression contributes to cell proliferation, migration, and invasion capability of ovarian cancer cells. Besides, we demonstrated that LINC00909 functions as a competing endogenous RNA (ceRNA) of MRC2 mRNA by sponging miR-23-3p, and thereby promotes epithelial-to-mesenchymal transition (EMT) of ovarian cancer cells. Therefore, we highlight that the LINC00909/miR-23b-3p/MRC2 axis is implicated in the pathogenesis of ovarian cancer, and serum LINC00909 may be a promising biomarker for the diagnosis of OC.

Dataset Information

Long non?coding RNA RP11?400N13.3 promotes the progression of colorectal cancer by regulating the miR?4722?3p/P2RY8 axis.

Publications

Long non‑coding RNA RP11‑400N13.3 promotes the progression of colorectal cancer by regulating the miR‑4722‑3p/P2RY8 axis.

Similar Datasets

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