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Draft genome sequence data of Erysipelothrix rhusiopathiae vaccine strain VR-2.

ABSTRACT: Data on the draft genome sequence of Erysipelothrix rhusiopathiae strain VR-2 is presented in this report. E. rhusiopathiae strain VR-2 is a commercial attenuated vaccine widely used in Russia and a number European countries for immunization of pigs against swine erysipelas. The draft genome sequence of 1,704,727?bp in length included 1415 protein sequences, 50 tRNA genes and 3 rRNA genes according NCBI Prokaryotic Genomes Automatic Annotation Pipeline results. The draft genome sequence data of E. rhusiopathiae strain VR-2 is available in GenBank under the accession nos. RJTK00000000.1, PRJNA504614 and SAMN10395786 for Genome, Bioproject and Biosample, respectively. The obtained sequence data may be helpful for searching genetic markers of VR-2, aimed to develop assays to discriminate between field isolates and this vaccine strain of E. rhusiopathiae.

SUBMITTER: Kovalchuk S

PROVIDER: S-EPMC7551983 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Draft genome sequence data of Erysipelothrix rhusiopathiae vaccine strain VR-2.

Kovalchuk Svetlana S Babii Anna A

Data in brief 20200928

Data on the draft genome sequence of Erysipelothrix rhusiopathiae strain VR-2 is presented in this report. E. rhusiopathiae strain VR-2 is a commercial attenuated vaccine widely used in Russia and a number European countries for immunization of pigs against swine erysipelas. The draft genome sequence of 1,704,727 bp in length included 1415 protein sequences, 50 tRNA genes and 3 rRNA genes according NCBI Prokaryotic Genomes Automatic Annotation Pipeline results. The dr ...[more]

PMID: 33083506

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Project description:BACKGROUND: Erysipelothrix rhusiopathiae is the causative agent of animal erysipelas and, to a fewer occurrences, human erysipeloid. It is ubiquitous in nature and commensal in diverse species of animals, wild or domestic, from mammals and birds to reptiles and fish. Mechanisms of its virulence and pathogenicity are poorly understood. RESULTS: Making use of the complete genome sequencing of E. rhusiopathiae strain SY1027 and comparative genome analysis between the three highly pathogenic strains (SY1027, Fujisawa and ATCC19414), the genomic structure and putative functional elements, such as pathogenicity island (PAI)-like regions, potential virulence factors and horizontal transferring genes of the bacteria are identified. Strain SY1027 genome is 1,752,910 base pairs long, just 30 kilobases smaller than strain Fujisawa, with the same GC level of 36.36%. It contains 1,845 open reading frames (ORF) predicted by GLIMMER 3.02, of which 1,775 were annotated by PGAAP, 1,757 (~95.23%) were annotated by NCBI nr blast, 1,209 by COG database and 1,076 by KEGG database. 37 potential virulence factors were annotated in strain SY1027 by VFDB, while 19 (~51.35%) of them are common in the 2 strains, 7 of which are potentially related to antibiotic resistance and highly conserved (~98-100% match identity (ID)) amongst the three strains of E. rhusiopathiae and modestly homologous to other gastrointestinal tract-inhabiting Firmicutes (~40% match ID), e.g. Clostridium spp., Enterococcus spp. Genomic island- and pathogenicity island-like regions were also predicted, in which some showed association with tRNA and potential virulence factors. CONCLUSION: Complete genome sequencing of Erysipelothrix rhusiopathiae, the causative agent of animal erysipelas, was performed. Molecular identification of various genomic elements pave the way to the better understanding of mechanisms underlying metabolic capabilities, pathogenicity of swine erysipelas and prospective vaccine targets besides the widely used SpaA antigens.

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Draft genome sequence data of Erysipelothrix rhusiopathiae vaccine strain VR-2.

Publications

Draft genome sequence data of <i>Erysipelothrix rhusiopathiae</i> vaccine strain VR-2.

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