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Protein-crystal detection with a compact multimodal multiphoton microscope.

ABSTRACT: There is an increasing demand for rapid, effective methods to identify and detect protein micro- and nano-crystal suspensions for serial diffraction data collection at X-ray free-electron lasers or high-intensity micro-focus synchrotron radiation sources. Here, we demonstrate a compact multimodal, multiphoton microscope, driven by a fiber-based ultrafast laser, enabling excitation wavelengths at 775?nm and 1300?nm for nonlinear optical imaging, which simultaneously records second-harmonic generation, third-harmonic generation and three-photon excited ultraviolet fluorescence to identify and detect protein crystals with high sensitivity. The instrument serves as a valuable and important tool supporting sample scoring and sample optimization in biomolecular crystallography, which we hope will increase the capabilities and productivity of serial diffraction data collection in the future.

SUBMITTER: Cheng QD

PROVIDER: S-EPMC7553921 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature

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Protein-crystal detection with a compact multimodal multiphoton microscope.

Cheng Qing-di QD Chung Hsiang-Yu HY Schubert Robin R Chia Shih-Hsuan SH Falke Sven S Mudogo Celestin Nzanzu CN Kärtner Franz X FX Chang Guoqing G Betzel Christian C

Communications biology 20201013 1

There is an increasing demand for rapid, effective methods to identify and detect protein micro- and nano-crystal suspensions for serial diffraction data collection at X-ray free-electron lasers or high-intensity micro-focus synchrotron radiation sources. Here, we demonstrate a compact multimodal, multiphoton microscope, driven by a fiber-based ultrafast laser, enabling excitation wavelengths at 775 nm and 1300 nm for nonlinear optical imaging, which simultaneously records second-harmonic genera ...[more]

PMID: 33051587

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